Fig. 3. Neuronal cell survival after induction of status epilepticus (SE) in the kainic acid (KA) model. Brain sections were stained against mouse anti-neuron-specific nuclear protein (NeuN) to evaluate neuronal cell survival (A). The KA treatment induced a significant decrease, compared to control group (CTRL), in the number of NeuN-positive cells in the CA3 stratum pyramidalis (CA3 Py, subregion B) (B), hilus of the dentate gyrus (DH) (C), subiculum (Sub) (D), layer III of the medial entorhinal cortex (MEnt L.III) (E), nucleus reuniens (Re) (F) and anterior olfactory nucleus (AOP) (G). This significant decrease in the number of NeuN-positive cells is present 3 (8 weeks of age) and 64 (17 weeks of age) days after the KA administration. Quantification was performed using ImageJ. Statistical analysis was performed using a one-way ANOVA followed by all pairwise multiple comparison procedures (Holm-Sidak test). Data are shown as mean ± SEM.* p<0.05, ** p<0.01, *** p<0.001; Scale bar: 100 μm. WK, week.